Screening for site-specific proteases
نویسندگان
چکیده
منابع مشابه
A genetic screen for the isolation and characterization of site-specific proteases.
Site-specific proteolysis is an important regulatory mechanism in basic cellular and viral processes. Using the protease of the HIV as a model, a genetic system has been developed for the isolation and characterization of site-specific proteases. The system utilizes the well defined bacteriophage lambda regulatory circuit where the viral repressor, cI, is specifically cleaved to initiate the ly...
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Site-specific proteases, which catalyze cleavage of peptide bonds in specific amino acid sequences of target proteins, play important roles in various biological events of many living organisms. In humans, disruption in regulation of these site-specific proteases can lead to pathological consequences. Here, we report a simple in vitro assay for enzymatic activities of site-specific proteases. T...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2000
ISSN: 1087-0156,1546-1696
DOI: 10.1038/71793